Table 2. Surface area of epidermal cells in the adaxial and abaxial sides of whorls 1 and 2 organs of wild-type plants (WT) and a Type I transgenic plants showing a severe morphological alteration (LiCrB25) a.
| Floral organ | Plant stain | Basal position | Middle position | Distal position | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Adaxial side (mm2) | Abaxial side (mm2) | Adaxial/abaxial sides | Adaxial side (mm2) | Abaxial side (mm2) | Adaxial/abaxial sides | Adaxial side (mm2) | Abaxial side (mm2) | Adaxial/abaxial sides | ||
| Whorl 1 organ | WT | 111.0±3.9 | 97.3±3.1 | 1.1±0.1 | 87.1±4.3 | 64.4±4.6 | 1.4±0.0 | 75.5±3.4 | 69.1±1.5 | 1.1±0.0 |
| LiCrB25 | 51.7±1.6 * | 86.3±8.0 ns. | 0.6±0.1 * | 56.2±4.9 * | 70.7±8.4 ns. | 0.8±0.0 * | 58.5±1.2 * | 68.3±3.5 ns. | 0.9±0.1 * | |
| Whorl 2 organ | WT | 120.7±2.2 | 96.2±5.7 | 1.3±0.1 | 97.4±7.7 | 87.2±8.5 | 1.1±0.0 | 93.0±2.5 | 76.9±5.9 | 1.2±0.1 |
| LiCrB25 | 67.3±6.3 * | 74.6±7.6 ns. | 0.9±0.1 * | 52.6±3.2 * | 57.3±4.9 * | 0.9±0.0 * | 53.8±2.4 * | 67.7±3.4 ns. | 0.8±0.1 * | |
a Surface area of epidermal cells was measured using the image analysis software ImageJ. Values represent the mean ± standard error of three replicates each consisted of ten epidermal cells.
* and ns. indicate significant and non-significant, respectively, compared with WT (P<0.05 by Turkey-Kramer’s test).