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. 2020 Jul 22;16(7):e1008920. doi: 10.1371/journal.pgen.1008920

Fig 6. Loss of Nf1 across combinations of distributed circuits drives elevated grooming.

Fig 6

(A) Grooming duration in flies with Nf1 knockdown in one or both sets of neurons labeled by the R13F10 (R13) and R11B06 (R11) Gal4 drivers. n = 20; Kruskal-Wallis; *p < 0.05 compared to UAS and Gal4 controls, n.s. not significant compared to UAS or Gal4 controls (Dunn/Sidak). (B) Maximum-intensity projection showing the expression of R13F10-Gal4 (green) and anti-neuroglian immunostaining (magenta) in a 48-hr pupal brain. (C) 48-hr pupal VNS, as in panel B. (D) GFP channel of VNS shown in panel C. (E) Maximum-intensity projection showing the expression of R11B06-Gal4 (green) and anti-neuroglian immunostaining (magenta) in a 48-hr pupal brain. (F) 48-hr pupal VNS, as in panel B. (G) GFP channel of the VNS shown in panel F.