Extended Data Fig. 8 |. Single-particle cryo-EM analysis of DGAT1 with acyl-CoA substrate.

a, Local resolution of the final cryo-EM map of DGAT1 (shown as sliced view) complex with an intact oleoyl-CoA molecule. b, FSC curves: gold-standard FSC curve between the two half maps with indicated resolution at FSC = 0.143 (red); FSC curve between the atomic model and the final map with indicated resolution at FSC = 0.5 (blue); FSC curve between half map 1 (orange) or half map 2 (green) and the atomic model refined against half map. c, Cutaway views of angular distribution of particle images included in the final 3D reconstruction. d-f, Similar to a-c, but shown the cryo-EM analysis of the map with a broken oleoyl-CoA density. Note in e only FSC curve between half maps was calculated. g, Cryo-EM densities that with the intact oleoyl-CoA density (shown in a-c) superimposed with the atomic model for individual transmembrane helices similar to that shown in Extended Data Fig. 3d. Maps are contoured at 4 σ.