KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| SSRP1 | Santa Cruz Biotechnology | Cat#sc-74536; RRID:AB_1129655 |
| SPT16 | Dr. Hasan Yardimci | N/A |
| Purified Mouse IgG1 | BioLegend | Cat#400101 |
| GAPDH | Cell Signaling Technology | Cat#2118; RRID:AB_561053 |
| SPT16 | Cell Signaling Technology | Cat#12191; RRID: AB_2732025 |
| Cas9 | Novus Biologicals | Cat#7A9-3A3 |
| SSRP1 | BioLegend | Cat#609701; RRID:AB_315730 |
| PE anti-human CD25 | BioLegend | Cat#302606; RRID:AB_314276 |
| FITC anti-human CD55 | BioLegend | Cat#311306; RRID:AB_314863 |
| FITC anti-human CD59 | BioLegend | Cat#304706; RRID:AB_2076126 |
| PE Mouse IgG1 κ isotype control | BioLegend | Cat#400112; RRID:AB_2847829 |
| FITC Mouse IgG1, κ Isotype control | BioLegend | Cat#400108 |
| FITC Mouse IgG2a, κ isotype control | BioLegend | Cat#400208 |
| H3K9me2 | Abcam | Cat#ab1220; RRID:AB_449854 |
| H3K27ac | Abcam | Cat#ab4729; AB_2118291 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| S. pyogenes Cas9 | QB3-Berkeley Macrolab | N/A |
| S. pyogenes dCas9 | QB3-Berkeley Macrolab | N/A |
| S. pyogenes Cas9-BirA* | This manuscript | N/A |
| S. pyogenes dCas9-BirA* | This manuscript | N/A |
| Recombinant Human FACT | Dr. Danny Reinberg | N/A |
| Critical Commercial Assays | ||
| HiScribe™ T7 High Yield RNA Synthesis Kit | New England Biolabs | Cat#E2040S |
| Deposited Data | ||
| Amplicon-NGS Raw Sequence Reads | This manuscript | SRA:PRJNA634106 |
| Oligonucleotides | ||
| SMARTpool ON-TARGETplus SUPT16H siRNA | Horizon Discovery | Cat#L-009517-00 |
| ON-TARGETplus SSRP1 siRNA | Horizon Discovery | Cat#J-011783-07 |
| ON-TARGETplus Nontargeting Pool | Horizon Discovery | Cat#D-001810-10 |
| Recombinant DNA | ||
| pMJ915 | Lin et al., 2014 | Addgene Plasmid #69090 |
| pCRISPRia-v2 | Horlbeck et al., 2016 | Addgene Plasmid #84832 |
| Software and Algorithms | ||
| Cortado Amplicon-NGS Analysis | https://github.com/staciawyman/cortado | N/A |