Table 2.
Primer name |
Sequence of primer |
References |
---|---|---|
Tn5ext |
5′-TATTGCTGAAGAGCTTGGC-3′ |
This study |
arb1 |
5′-GGCCACGCGTCGACTAGTACNNNNNNNNNN-3′ |
[21] |
Tn5int |
5′-GCATCGCCTTCTATCGCCTTC −3’ |
This study |
arb2 |
5′-GGCCACGCGTCGACTAGTAC-3′ |
[21] |
prMutS1 |
5′-TATCAAAACGACATCAAAGCCGTTGC-3′ |
This study |
prMutS2 |
5′-TTAGTGGAACTGGTTCATGGTGTT-3′ |
This study |
prMutD1 |
5′-ATGCCCGCTCAACCGACC-3′ |
This study |
prMutD2 |
5′-TCACGGGGTCCTTCT-3′ |
This study |
prRegBam |
5′-AGGATCCGGAGGGTTGAGCAGACAT-3′ |
This study |
prRegEco |
5′-AGAATTCCGCGTGTGCTCGTTGCG-3′ |
This study |
prLigBam |
5′-AGGATCCTTGGGGTTGCTGCTGTCT-3′ |
This study |
prLigEco |
5′-AGAATTCGGAGGGTTGAGCAGACAT-3′ |
This study |
prIsocitBam |
5′-AGGATCCAGAACGGTTTCGCCCG-3′ |
This study |
prIsocitEco |
5′-AGAATTCTTTCAGAGCGGCAACGG-3′ |
This study |
KnRegXho |
5′-ACTCGAGGAAATCTCGCGGATCACCG-3′ |
This study |
KnRegXba |
5′-ATCTAGATCATCGACAGCCAGTCACGG-3′ |
This study |
prDehEco |
5′-AGAATTCACCGGTGGGTTGTCGACT-3′ |
This study |
prDehBam |
5′-AGGATCCGCCAGCAACTGGTGTT-3′ |
This study |
ΔReg mut |
5′-ATCACCGACCAATCGCCTTCG-3′ |
This study |
prRegSensBam |
5′-AGGATCCGAGCAGGCGATCCAG-3′ |
This study |