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(a) Muscle fiber cross-sectional area (CSA) and (b) density of hatched chicks fed in ovo with nicotinamide riboside (NR) during embryogenesis. At day 10 of incubation, embryos were injected with 100 µL of 0.9% saline containing 0 or 250 mM NR in the yolk sac or albumen. Immunohistochemistry and muscle fiber morphometric data collection were conducted as described by Noel et al. (2016). a,bMeans with different superscripts differ (P < 0.05).
