Table 1.

Body and pectoralis major morphometrics of hatched chicks injected in ovo with nicotinamide riboside during embryogenesis

Nicotinamide riboside dose1	0 mM		250 mM			P-value2
Injection location3	Albumen	Yolk	Albumen	Yolk	SEM	TRT	LOC	TRT × LOC
n	27	32	30	24
Weight, g	42.69	42.99	43.19	44.02	6.40	0.17	0.31	0.63
Body measurements
Dimensions, mm
Crown-rump length	95.60	96.93	96.14	89.36	3.70	0.12	0.23	0.07
Head width	15.40	15.74	15.56	15.45	0.40	0.67	0.48	0.21
Head length	21.79	21.86	21.29	21.73	0.19	0.42	0.51	0.63
Head circumference	32.38	32.08	32.14	31.49	1.60	0.70	0.66	0.87
Chest length	22.01	21.96	21.98	22.68	0.55	0.36	0.39	0.29
Chest width	16.90	17.35	16.57	17.69	0.33	0.98	0.01	0.28
Heart weight, g	0.36	0.36	0.35	0.36	0.02	0.75	0.55	0.24
Liver weight, g	1.09	1.08	1.05	1.11	0.06	0.64	0.36	0.19
Pectoralis major measurements
Weight, g	0.13a	0.13a	0.14a	0.18b	0.01	<0.01	<0.01	<0.01
Dimensions, mm
Length	17.55a	17.04a	18.68b	20.74c	0.43	<0.01	0.06	<0.01
Width	4.59	4.62	4.81	5.21	0.22	<0.01	0.13	0.20
Depth	2.27	2.32	2.43	2.65	0.09	<0.01	0.09	0.30

^abcTreatments with different superscripts within a row differ (P < 0.05).

¹100 µL of 0.9% saline containing 0 or 250 mM nicotinamide riboside injected at day 10 of incubation.

²TRT denotes nicotinamide riboside treatment main effect; LOC denotes injection location main effect.

³Treatments were injected into either the yolk sac or albumen of the egg.