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. Author manuscript; available in PMC: 2021 Aug 30.
Published in final edited form as: Prog Neuropsychopharmacol Biol Psychiatry. 2020 May 6;102:109961. doi: 10.1016/j.pnpbp.2020.109961

Fig. 3.

Fig. 3.

Purification of PCocSH for animal studies. (A) Size exclusion-HPLC analysis and (B) enzymatic activity of fractions collected during SE-HPLC analysis. Monomer, dimer, and tetramer fractions are indicated with 1°, 2°, and 4° respectively. Blue dextran (2000 kDa), beta amylase (200 kDa), albumin (66 kDa) and carbonic anhydrase (29 kDa) were included as size references and respective peak elution times are indicated by arrows. Samples for enzymatic activity were collected from the HPLC (0.5 mL) every 1 min. (C) Silver stain and (D) western blot of pooled, purified preparations of PCocSH used in animal studies alongside uninfiltrated wildtype (WT) Nicotiana benthamiana control plant extract. Please note that monomeric plant-derived BChE is glycosylated with high mannose glycans thereby raising its apparent molecular mass.