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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Cell Stem Cell. 2020 May 26;26(6):938–950.e6. doi: 10.1016/j.stem.2020.04.020
0.2μL barcodelet-specific cDNA Library
7.5μL 2X NEBNext mastermix (NEB)
0.375 μL 20 uM Bcdltr2_ixN701_fw
0.375 uL 20 uM PE1
5.8 μL H20
0.75 μL 20X EvaGreen Dye (Biotium)