| 20μL | cDNA Library |
| 50μL | 2X NEBNext MasterMix |
| 2.5 μL | 20 uM Bcdltr2_ixX_fw (use different indexed primer for each sample) |
| 2.5 uL | 20 uM PE1 |
| 25 μL | H20 |
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