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. 2020 Jul 22;9:e55415. doi: 10.7554/eLife.55415

Figure 10. AGK2-treated splenocytes demonstrate increased propensity toward mycobacterial killing and transfer Mtb-specific protective immunity in naive animals.

Figure 10.

(A) Schematic representation of the adoptive transfer experiment. Splenocytes isolated from infected and AGK2-treated C57BL/6 mice were analyzed for (B and C) apoptosis by Annexin V staining and (D and E) ROS production via cellROX (see Materials and methods). (F) Splenocytes isolated from infected and AGK2 treated mice were co-cultured with peritoneal macrophages infected with GFP expressing H37Rv. 48 hr pi, percentage of infected cells were analyzed by flow cytometry. CD4+ and CD8+ T cells purified from the spleen of infected and AGK2-treated mice (G) were intravenously transferred into Rag1-/- mice followed by challenge with aerosolized Mtb. (H) CFU enumeration 21 days after adoptive transfer. Data is representative of two independent experiments performed with four to five mice in each group. Data is represented as mean ± SD (n = 5). H shows combined data from two independent experiments (n = 9). *p<0.05, ***p<0.0005.

Figure 10—source data 1. AGK2-treated splenocytes transfer Mtb-specific protective immunity in naive animals.
Source data for Figure 10C, E, F and H.