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. 2020 Jul 22;9:e55415. doi: 10.7554/eLife.55415

Figure 2. SIRT2 translocate to the nucleus and modulates histone deacetylations and cellular signaling during Mtb infection.

Figure 2.

(A) Endogenous SIRT2 was detected by immunofluorescence in mouse peritoneal macrophages uninfected or infected with H37Rv for 4 hr. (B) Uninfected macrophages or macrophages infected with H37Rv for 24 hr were fractionated for cytosol and nucleus followed by immunoblotting for the indicated proteins. Mouse peritoneal macrophages, pre-treated with AGK2 for 2 hr were infected H37Rv followed by AGK2 treatment for 24 hr. Acetylation levels of H3K18 were checked by (C) immunoblotting and (D) intracellular staining followed by flow cytometry. (E) Acetylated α-tubulin levels in uninfected and Mtb-infected cells with or without AGK2 treatment at 24 hr pi. (F) Mouse peritoneal macrophages, pre-treated with AGK2 for 2 hr were infected with H37Rv for 2 hr. Phosphorylation status of the indicated proteins was checked by immunoblotting. Data shown is representative of at least two independent experiments performed in triplicates. Each bar represents mean ± SD (n = 3). *p<0.05, **p<0.005.

Figure 2—source data 1. SIRT2 migrates to the nucleus upon Mtb infection.
Source data for Figure 2B and D.