Figure 2.

Stacked UHPLC-UV chromatograms (A) and ¹H NMR spectra (B; 400 MHz, DMSO-d₆) of the E. sagittatum extract replicates, n1-n3. (A) The reproducibility of the extraction process and, thus, of the phytochemical profiles were evaluated at 254 nm and 400 nm by measuring and comparing the peak area of each of the four regions of interest (ROIs) to the peak area of the entire chromatogram at 254 nm (and 400 nm for chlorophylls). Results are expressed as peak area percentages. (B) The reproducibility of ¹H NMR spectroscopy phytochemical fingerprints were evaluated by measuring and comparing the absolute integrals of each of the three ROIs to those of the full integral measured for the entire spectrum (2.6 – 10.0, 0.0 – 2.4 ppm, excluding solvent range). Results are also expressed as percentages relative to all quantifiable ¹H NMR signals.