Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | Tudor | R. Boswell; similar stock now available from BloomingtonDrosophila Stock Center | BDSC:1735; FBst0001735;RRID:BDSC_1735 |
FlyBase Genotype:tud1 bw1 sp1/CyO |
| Genetic reagent (D. melanogaster) | Δ325/TM3; Sb ry (SP-knockout line) | Gift from Eric Kubli | ||
| Genetic reagent (D. melanogaster) | Δ130/TM3; Sb ry (deficiency line) | Gift from Eric Kubli | ||
| Genetic reagent (D. melanogaster) | ProtB-eGFP(X); TM3/TM6 | Gift from Scott Pitnick | ||
| Genetic reagent (D. melanogaster) | ProtB-DsRed | Gift from Scott Pitnick | ||
| Antibody | anti-SP (rabbit polyclonal) |
Wolfner lab | IF (1:200), WB (1:2000) |
|
| Antibody | anti-CG1656 (rabbit polyclonal) |
Wolfner lab | IF (1:100), WB (1:1000) |
|
| Antibody | anti-CG1652 (rabbit polyclonal) |
Wolfner lab | IF (1:50), WB (1:500) |
|
| Antibody | anti-CG9997 (rabbit polyclonal) |
Wolfner lab | IF (1:50), WB (1:1000) |
|
| Antibody | IgG (H+L) Goat anti-Rabbit, Alexa Fluor 488 (goat anti-rabbit polyclonal) |
Invitrogen | Cat. # A11008 RRID:AB_143165 |
IF (1:300) |
| Antibody | IgG (H+L) Goat anti-Rabbit, Alexa Fluor 594 (goat anti-rabbit polyclonal) |
Invitrogen | Cat. # A11012 RRID:AB_2534079 |
IF (1:300) |
| Antibody | anti-Antares (rabbit polyclonal) |
Wolfner lab | WB (1:500) | |
| Antibody | anti-seminase (rabbit polyclonal) |
Wolfner lab | WB (1:1000) | |
| Antibody | anti-CG17575 (rabbit polyclonal) |
Wolfner lab | WB (1:1000) | |
| Antibody | Anti-actin (mouse monoclonal) |
Millipore Corp | Cat# MAB1501 RRID:AB_2223041 |
WB (1:3000) |
| Antibody | Peroxidase AffiniPure Goat Anti-Rabbit IgG (goat anti-rabbit polyclonal) |
Jackson Research | Code#111-035-003 RRID:AB_2313567 |
WB (1:2000) |
| Antibody | Peroxidase AffiniPure Goat Anti-Mouse IgG (goat anti-mouse polyclonal) | Jackson Research | Code#115-035-003 RRID:AB_10015289 |
WB (1:2000) |
| Other | DAPI stain | Invitrogen | Cat. # PI62247 | (1 µg/mL) |
| Other | Poly-L-Lysine (0.1 % w/v in H2O) |
Sigma | P8920-100ML | 0.01% w/v in H2O |
| Other | Albumin from Bovine Serum (BSA) | Sigma | A9418-50G | 5% in 1X PBS |
| Other | CitiFluor Mountant Solution | Electron Microscopy Sciences | Cat. #17970–100 | |
| Software, Algorithm | Graph Pad Prism | RRID:SCR_002798 | Version 6.01 |