Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Saccharomyces cerevisiae) | Various | This paper | NCBITaxon:4932 | SeeSupplementary file 1a |
| Antibody | Anti-c-myc beads (mouse monoclonal) | Thermo Fisher Scientific | Cat # 88842 | 50 µL per IP |
| Antibody | Anti-H3K79me3 (rabbit polyclonal) | Diagenode | Cat # C15410068 | 5 µL per IP |
| Antibody | Anti-ERα (rabbit polyclonal) | Santa Cruz Biotechnology | Cat # sc-8002, RRID:AB_627558 | 25 µL per IP |
| Antibody | Anti-Hexokinase (Rabbit polyclonal) | Rockland | Cat # 100–4159, RRID:AB_219918 | (1:20,000) |
| Antibody | Anti-V5 (Mouse monoclonal) | Thermo Fisher Scientific | Cat # R960-25, RRID:AB_2556564 | (1:2,500) |
| Antibody | IRDye 800CW anti-mouse (Goat polyclonal) | Li-Cor | Cat # 926–32210, RRID:AB_621842 | (1:20,000) |
| Antibody | IRDye 680RD anti-rabbit (Goat polyclonal) | Li-Cor | Cat # 926–68070, RRID:AB_10956588 | (1:20,000) |
| Recombinant DNA reagent | HML* | This paper | Mutated allele of HML | |
| Recombinant DNA reagent | SIR3-EBD | This paper | Fusion protein of Sir3 and EBD of mammalian ERα | |
| Sequence-based reagent | Various oligonucleotides | This paper | qPCR primers | SeeSupplementary file 1b |
| Sequence-based reagent | smRNA-FISH probes | Biosearch Technologies | SeeSupplementary file 1c | |
| Commercial assay or kit | RNEasy Mini Kit | Qiagen | Cat # 74104 | |
| Commercial assay or kit | Qiaquick PCR purification kit | Qiagen | Cat # 28104 | |
| Commercial assay or kit | NEBNext Ultra II Library prep kit | NEB | Cat # 37645L | |
| Commercial assay or kit | Superscript III reverse transcriptase kit | Thermo Fisher Scientific | Cat # 18080044 | |
| Commercial assay or kit | DyNamo HS SYBR Green qPCR kit | Thermo Fisher Scientific | Cat # F410L | |
| Peptide, recombinant protein | Catalase | Sigma-Aldrich | Cat # C3515 | |
| Peptide, recombinant protein | Proteinase K | NEB | Cat # P8107S | |
| Peptide, recombinant protein | Glucose oxidase | Sigma-Aldrich | Cat # G2133 | |
| Peptide, recombinant protein | Zymolyase-100T | VWR | Cat # IC320932 | |
| Peptide, recombinant protein | Protein A beads | Thermo Fisher Scientific | Cat # 10002D | |
| Peptide, recombinant protein | α-factor peptide | Elim Bio | ||
| Peptide, recombinant protein | Uracil-DNA Glycosylase | Thermo Fisher Scientific | Cat # EN0362 | |
| Peptide, recombinant protein | Micrococcal nuclease | Worthington Biochemical | Cat # LS004798 | |
| Commercial assay or kit | RNase-Free DNase Set | Qiagen | Cat # 79254 | |
| Chemical compound, drug | Nocodazole | Sigma-Aldrich | Cat # M1404 | |
| Chemical compound, drug | β-estradiol | Sigma-Aldrich | Cat # E8875 | |
| Chemical compound, drug | cOmplete EDTA-free protease inhibitor | Simga-Aldrich | Cat # 11873580001 | |
| Chemical compound, drug | 3-indoleacetic acid | Sigma-Aldrich | Cat # I2886 | |
| Software, algorithm | SAMtools | doi:10.1093/bioinformatics/btp352 | RRID:SCR_002105 | |
| Software, algorithm | Ggplot2 | doi:10.1007/978-0-387-98141-3 | RRID:SCR_014601 | |
| Software, algorithm | Bowtie2 | doi:10.1038/nmeth.1923 | RRID:SCR_005476 | |
| Software, algorithm | FISH-quant | doi:10.1038/nmeth.2406 | ||
| Software, algorithm | FlowJo | BD Life Sciences | RRID:SCR_008520 |