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. 2020 Jul 23;9:e58571. doi: 10.7554/eLife.58571

Table 1. Yeast strains.

Strain name Genotype Purpose
YDR125 W303-1a MATa ade2-1 trp1-1 can1-100 pep4::kanMX bar::hphNAT1 (hygromycinB) his3-11,15::P/Gal 1,10-GAL4 (HIS3) leu2-3,112::P/Gal 1,10-CBP-POB3++ (LEU2) ura3-1::P/Gal 1,10-SPT16++ (URA3) Overexpression and purification of FACT
YSA11 W303-1a MATa ade2-1 can1-100 pep4::kanMX bar1::hphNAT1 (hygromycinB) his3-11,15::GAL4-P/Gal1,10-CDT1 (HIS3) trp1-1::MCM5-P/Gal1,10-MCM4 (TRP1) leu2-3,112::MCM7-P/Gal1,10-MCM6 (LEU2) ura3-1::MCM2-TEV-P/Gal1,10-FLAG-MCM3 (URA3) Overexpression and purification of Cdt1·Mcm2-72-TEV
YSA27 W303-1a MATa ade2-1 can1-100 pep4::kanMX bar1::hphNAT1 (hygromycinB) his3-11,15::GAL4-P/Gal1,10-CDT1 (HIS3) trp1-1::MCM5-P/Gal1,10-MCM4 (TRP1) leu2-3,112::MCM7-P/Gal1,10-MCM6 (LEU2) ura3-1::MCM2-2A-P/Gal1,10-FLAG-MCM3 (URA3) Overexpression and purification of Cdt1·Mcm2-72-2A
YSA35 W303-1a MATa ade2-1 ura3-1 trp1-1 can1-100 pep4::kanMX bar::hphNAT1 (hygromycinB) his3-11,15::P/Gal 1,10-GAL4 (HIS3) leu2-3,112::CDC7-myc-P/Gal 1,10-DBF4-ybbR-FLAG (LEU2) Overexpression and purification of DDK