Figure 2.
Anti-Gal-assisted vaccines are effective in blunting fentanyl effect and sequestering the drug in blood. Fentanyl citrate dose-response curves were measured in (A) hot plate and (B) tail flick antinociception tests (n = 7 or 8). %MPE = percentage of the maximum possible drug effect. (C) and (D), ED50 values determined in the tests and fold change with respect to the control groups are shown. Fentanyl concentrations in (E) serum and (F) homogenized brains of mice sacrificed 15 min after drug administration are shown as individual points and means, n = 4. Error bars denote SEM. ****P < 0.0001 versus control, Brown-Forsythe and Welch ANOVA test.