Figure 6. Chronic ethanol consumption reduced leukocyte rolling and adhesion in vivo and neutrophils chemotaxis ex vivo.

(A) Experimental design: after the chronic ethanol treatment, mice received an intracrostal injection of LPS. After 2 hr, mice cremaster from mouse was exposed to examine the microcirculation by intravital microscopy. Post capillaries venules were recorded. (B) Representative images from the recorded videos (please see the rich media). (C) Number of rolling cells and (D) Number of adherent cells were counted in the videos. (E) Ex vivo neutrophil chemotaxis. Experimental design: after ethanol treatment, BM-derived neutrophils were separated by density gradient and a chemotaxis assay in a Boyden chamber towards CXCL2 was performed. (F) Number of migrated neutrophils in 60 min. Data are presented as Mean ± SD (3 to 5 mice per group) *Significantly different (p<0.0028) in ANOVA test. Please, also see Figure 6—source data 1 and Figure 6-rich media videos.

Figure 6—video 1. Leukocyte rolling and adhesion in vivo-control mice + vehicle.

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After chronic ethanol treatment, mice received an intracrostal injection of LPS or vehicle (saline). After 2 hr, mice cremaster from mouse was exposed to examine the microcirculation by intravital microscopy. Post capillaries venules were recorded. Representative recorded videos from control mice + vehicle; (Figure 6—video 2) ethanol-treated mice + vehicle; (Figure 6—video 3) LPS-stimulated control mice and (Figure 6—video 4) LPS-stimulated ethanol treated mice.

Figure 6—video 2. Leukocyte rolling and adhesionin vivo-ethanol-treated mice + vehicle.

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Figure 6—video 3. Leukocyte rolling and adhesionin vivo-control mice + LPS.

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Figure 6—video 4. Leukocyte rolling and adhesionin vivo- ethanol-treated mice + LPS.

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