Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J | Multidisciplinary center for Biological Investigation on Laboratory Animal Science (CEMIB) -Unicamp | C57BL/6JUnib | |
| Genetic reagent (Aspergillus fumigatus) | Strain A1163 |
A. fumigatus CEA17 isolate (CEA10 derivative) and converted to pyrG+ via A. niger pyrG gene ectopic insertion Fedorova et al., 2008; Malacco et al., 2019. |
||
| Cell line (M. musculus) | Primary bone marrow neutrophils | This paper | C57BL/6JUnib | Freshly isolated from C57BL/6J (M. musculus) |
| Peptide, recombinant protein | Recombinant murine MIP-2 (CXCL2) | PeproTech | Cat# 250–15 | Chemotaxis (20 ng/ml) |
| Antibody | Purified NA/LE CD16/CD32 Clone 2.4G2 - FC Block (Rat monoclonal) | BD Biosciences | Cat# 553140 | FACS (1:100) |
| Antibody | anti-CD3-FITC (Rat monoclonal) | BD Biosciences | Cat# 555274 | FACS (1:100) |
| Antibody | anti-CD4-APC (Rat monoclonal) | BD Biosciences | Cat# 553051 | FACS (1:200) |
| Antibody | anti-IL-17a-PE (Rat monoclonal) | BD Biosciences | Cat# 559502 | FACS (1:100) |
| Antibody | anti-Ly6G-BV421 (Rat monoclonal) | BD Biosciences | Cat# 562737 | FACS (1:50) |
| Antibody | anti-CXCR2-PE (Rat monoclonal) | R and D Systems | Cat# FAB2164P | FACS (1:10) |
| Antibody | anti-CD62L-APC (Rat monoclonal) | BD Biosciences | Cat# 553152 | FACS (1:100) |
| Antibody | anti-CD11b-FITC (Rat monoclonal) | BD Biosciences | Cat# 553310 | FACS (1:100) |
| Commercial assay or kit | Mouse TNF-a ELISA kit | R and D Systems | Cat# DY410 | |
| Commercial assay or kit | Mouse IL-1b ELISA kit | R and D Systems | Cat# DY401 | |
| Commercial assay or kit | Mouse CXCL1 ELISA kit | R and D Systems | Cat# DY453 | |
| Commercial assay or kit | Mouse CXCL2 ELISA kit | R and D Systems | Cat# DY452 | |
| Commercial assay or kit | Mouse IL-17 ELISA kit | R and D Systems | Cat# DY421 | |
| Commercial assay or kit | Mouse IL-10 ELISA kit | R and D Systems | Cat# DY417 | |
| Software, algorithm | Prism | GraphPad | ||
| Software, algorithm | FlowJo | BD |