Table 2.
Sequences of the target genes in all FLZ-R (n = 4) and FLZ-SDD isolates (n = 7), and randomly selected FLZ-S isolates (n = 15).
| Strain no. | FLZ (µg/ml) | VRZ (µg/ml) | ITZ (µg/ml) | MRR1 | TAC1 | UPC2 | Genotype |
|---|---|---|---|---|---|---|---|
| Fluconazole-control isolates (n = 12) | |||||||
| N8 | 0.5 | 0.125 | 0.5 | A87T, V133A, M1022I, T1042N, T1044N, I1130M | WT | A251T, Q289L, G392E | G5 |
| N15 | 0.5 | 0.125 | 0125 | WT | L278S | N98S, L158V | G2 |
| N71 | 0.125 | 0.016 | 0.125 | S523F, K757E | L278S, D350N, F470C, D790N | G392E | MG |
| N104 | 1 | 0.25 | 0.125 | M1022I, T1042N, T1044N, D1092E, I1130M | L278S | WT | G4 |
| N147 | 0.5 | 0.031 | 0.125 | M1022I, T1042N, T1044N, D1092E, I1130M | L278S | WT | G4 |
| N195 | 0.25 | 0.031 | 0.125 | S523F, K757E | L278S, D350N, F470C, D790N | A297S*, G392E | G4 |
| N210 | 0.125 | 0.062 | 0.125 | WT | L278S | N98S, L158V, A251T | G2 |
| SU-221 | 0.25 | 0.015 | 0.06 | A87T), V133A, M1022I, T1042N, T1044N, D1092E, I1130M | L278S, F470C | WT | G5 |
| SU-267 | 0.25 | 0.125 | 0.125 | WT | L278S, F470C, S884G | N98S, L119F, A147T, L158V | MG |
| 10BC | 1 | 0.5 | 0.5 | I408T, M1022I, T1042N, T1044N, D1092E, I1130M | L278S, F470C, D790N, D790N | A251T, Q289L, A297S* | G6 |
| 24BC | 0.5 | 0.5 | 0.125 | S523F, K757E | L278S, F470C, D790N | G392E, T560N | G6 |
| 115-1BC | 0.5 | 0.125 | 0.125 | A87T, V133A, M1022I, T1042N, T1044N, I1130M | WT | A251T, Q289L | G5 |
| Fluconazole-susceptible isolates (n = 3) | |||||||
| N26 | 2 | 0.5 | 0.125 | S523F, K757E | L278S, D350N, F470C, D790N | A297S *, G392E | G6 |
| SU-235 | 2 | 0.125 | 0.125 | WT | L278S | N98S, L158V, A251T | G2 |
| 85BC | 2 | 0.25 | 0.125 | WT | L278S | N98S, L158V | G2 |
| Fluconazole-susceptible dose-dependent isolates (n = 7) | |||||||
| SU-239 | 4 | 0.06 | 0.125 | WT | L278S | L158V, N98S, L158V, F571Y | G2 |
| N17 | 4 | 0.5 | 1 | S523F, K757E, I1130M | L278S, L340*, D350N, F470C, D790N | NA | |
| 75BC | 4 | 0.125 | 0.25 | S523F, K757E, I1130M | L278S | WT | G4 |
| 82BC | 4 | 1 | 0.25 | I1130M | WT | A263T | G4 |
| 107BC | 4 | 0.062 | 0.125 | S523F, K757E | L278S, F470C, D790N | WT | G3 |
| 113-1BC | 4 | 1 | 0.125 | I1130M | L278S, F470C | A251T, Q289L | G4 |
| 115-2 | 4 | 0.5 | 0.06 | A87T, V133A, M1022I, T1042N, T1044N, I1130M | WT | A251T, Q289L, G392E | G5 |
| Fluconazole-resistant isolates (n = 4) | |||||||
| 99BC | 8 | 1 | 0.5 | V133A, A647S, M1022I, T1044N, D1092E, I1130M | L278S, F470C | WT | G5 |
| 113-2BC | 8 | 0.062 | 0.25 | WT | L278S | N98S, L158V, N230S, T241A | G2 |
| 262E | 64 | 4 | 16 | M1022I, T1042N, T1044N, D1092E, I1130M | R47Q, N164I, L278S | Q340H, T381S | G4 |
| 527E | 8 | 1 | 0.5 | T255P | R47Q, N164I, L278S, F470C, D790N | A147T, A251T, Q289L | MG |
Underlined boldface amino acid substitutions were only identified in FLZ-R or FLZ-SDD isolates; asterisk-denoted boldface amino acids were exclusively identified in resistant isolates in previous studies; boldface italicized amino acids were exclusively found in susceptible isolates in the current and previous studies. All strains carried the ERG11 WT sequence, except for SU-239 (K90I) and SU-267 (I25A). FLZ, fluconazole; ITZ, itraconazole; VRZ, voriconazole.
*
Stop codon; NA, not amplified.