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. 2020 Jun 15;150(8):2077–2088. doi: 10.1093/jn/nxaa162

FIGURE 5.

FIGURE 5

Shear force modifies gene expression of glycocalyx-related molecules, antimicrobial peptide, and tight junction with Lactobacillus plantarumWCFS1 in intestinal epithelial Caco-2 cells. (A) GPC1, (B) HAS1, (C) HAS2, (D) HAS3, (E) EXT1, and (F) EXT2 gene expression of the glycocalyx under static conditions and shear force with L. plantarumWCFS1; (G) DEFB1, 1 antimicrobial peptide gene expression under static conditions and shear force with L. plantarumWCFS1; (H) TJP1 and (I) CLDN3 gene expression of the tight junction protein under static conditions and shear force with L. plantarumWCFS1. Results are presented as relative values normalized to the housekeeping gene GAPDH. Values were means ± SDs, n = 6. Asterisks indicate different from static: *P <0.05, **P <0.01. CLDN3, claudin 3; DEFB1, defensin β-1; EXT, exostosin glycosyltransferase; GPC1, glypican 1; HAS, hyaluronan synthase; TJP1, tight junction protein 1.