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. 2020 Jul 21;13:7045–7056. doi: 10.2147/OTT.S243552

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© 2020 Wang et al.

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TCF12 was lowly expressed in cells and tissues of PCa and regulated cell proliferation, invasion and migration of DU145 cells. (A) TCF12 expression in PCa tissues and paracancerous tissues was detected by Western blotting. (B) TCF12 expression in PCa cell lines (LNCap, DU145 and PC3) and normal prostate epithelial cells (RWPE-1) was detected by Western blotting. The DU145 cells were transfected with TCF12 overexpression vector, TCF12 siRNA and respective negative controls, following transfection for 48 h, (C) the TCF12 overexpression and inhibition efficiencies were detected by Western blotting, and cell proliferation (D), invasion (E) and migration (F) were detected with CCK-8 and Transwell assay. *p<0.05, **p<0.01.