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. 2020 Jul 21;13:7045–7056. doi: 10.2147/OTT.S243552

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© 2020 Wang et al.

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EMX2OS played synergy effect with FUS protein in DU145 cells. The DU145 cells were transfected with FUS overexpression vector alone or together with EMX2OS overexpression vector, or transfected with FUS siRNA alone or together with EMX2OS siRNA, following transfection for 48 h, (A) the FUS overexpression and inhibition efficiencies were detected by Western blotting, and the protein levels of PKG1 and PKG2 (B), and the cGMP concentration (C) in supernatant were detected by Western blotting and ELISA. Cell proliferation (D), migration (E) and invasion (F) were detected with CCK-8 and Transwell assay. *p<0.05, **p<0.01.