Fig. 1.

Study design. Large white pigs (30–35 kg) were allocated to eight periods of ischemia by left anterior descending occlusion for 20 (n = 10), 25 (n = 10), 30 (n = 10), 35 (n = 10), 40 (n = 24), 45 (n = 24), 50 (n = 24), or 60 (n = 10) min, followed by reperfusion. Animals were 1:1 randomized to receive either intravenous metoprolol (0.75 mg/kg) or saline 20 min after ischemia onset. MDCT scans were performed during the course of ischemia. Cardiac magnetic resonance scans were performed 7 days after reperfusion, and 45 days after reperfusion