. 2020 Jul 30;5(4):252–267. doi: 10.1016/j.synbio.2020.07.006

Table 1.

Commonly used strains, with genotypes. Citations indicate originally developed locations and/or application.

Strain	Genotype	Ref(s)
BL21-Rosetta (DE3)^a	F⁻ompT hsdS_B(r_B⁻ m_B⁻) gal dcm (DE3)^a pRARE (Novagen)	[49,50]
BL21-Rosetta2 (DE3)^a	F⁻ompT hsdS_B(r_B⁻ m_B⁻) gal dcm (DE3)^a pRARE2 (Novagen)	[8,33,51]
BL21-Star (DE3)^a	F⁻ompT hsdS_B(r_B⁻, m_B⁻) gal dcm rne131 (DE3)^a	[25,49,50]
BL21-Gold-dLac (DE3)^a	F⁻ompT hsdS_B(r_B⁻ m_B⁻) dcm gal (DE3)^aendA lacZYA	[14]
JS006	MG1655 araC lacI	[52,53]
A19	rna gdhA2 relA1 spoT metB1	[11,50,54,55]
KC1	A19 speA tnaA tonA endA sdaA sdaB met⁺	[56]
KC6	KC1 gshA	[57]
KG6-der.	KC6 rnb ackA⁺ef-tu⁺hchA⁺ibpA⁺ibpB⁺if-1⁺if-2⁺if-3⁺	[58]
KGK10	KC6 gorB trxB-HA	[[59], [60], [61]]
NMR1	A19 endA met⁺	[56]
NMR2	A19 speA tnaA tonA endA met⁺	[56]
NMR4	A19 recD endA met⁺	[62]
NMR5	A19 lambda phage<>recBCD met⁺	[62]
S30BL/Dna	BL21(DE3) dnaK/J⁺grpE⁺	[63]
S30BL/DsbC	BL21(DE3) dsbC⁺	[63]
S30BL/GroE	BL21(DE3) groEL/ES⁺	[63]
S30OB	F⁻ompT hsdS_B(r_B⁻ m_B⁻) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (Novagen)	[63]
S30OB/Dna	S30OB dnaK/J⁺grpE⁺	[63]
S30OB/DsbC	S30OB dsbC⁺	[63]
S30OB/GroE	S30OB groEL/ES⁺	[63]

Each of these strains is available with and without DE3 modifications, which enables induction of T7 polymerase. Some studies use DE3 strains while others do not.