Table 3.

Summary of CFE systems from non-E. coli prokaryotes and their applications.

Organism	Type	CFE Application	Proteins Made	Ref(s)
Bacillus megaterium	Gram positive	Prototyping RBSs, promoters	GFP, mCherry	[38]
Bacillus subtilis	Gram positive	Prototyping promoters	GFPmut3b, renilla luciferase	[39,40]
Clostridium autoethanogenum	Gram positive	Prototyping genetic parts	Luciferase, metabolic enzymes	[47]
Corynebacterium glutamicum	Gram positive	Prototyping promoters	eGFP	[40]
Escherichia fergusonii	Gram negative	Prototyping promoters	eGFP	[40]
Klebsiella oxytoca	Gram negative	Prototyping promoters	eGFP	[40]
Lactococcus lactis	Gram positive	Prototyping promoters	None (only transcription active)	[40]
Pantoea agglomerans	Gram negative	Prototyping promoters	eGFP	[40]
Pseudomonas fluorescens	Gram negative	Protein synthesis at low temperatures	GFP, apolipoprotein, pancreatic RNase, p37a, glucokinase, peptidases	[143]
Pseudomonas putida	Gram negative	Prototyping genetic parts for bioremediation	sfGFP	[40,45]
Salmonella enterica	Gram negative	Prototyping promoters	eGFP	[40]
Streptomyces species	Gram positive	Natural product biosynthesis	eGFP, sfGFP, metabolic proteins	[41,46,142,144,145]
Sulfolobus solfataricus	Archaeal thermophile	Elucidation of archaeal translation mechanisms	ORF 104, ORF 143	[146,147]
Sulfolobus tokodaii	Archaeal thermophile	Translation of single stranded DNA	Polyphenylalanine	[148]
Thermococcus kodakaraensis	Archaeal thermophile	Production of thermostable proteins	Chitinase	[149]
Thermus thermophilus	Gram negative	Studying mechanisms of thermophilic protein synthesis	Polyphenylalanine	[148,150]
Vibrio natriegens	Gram negative	High protein yields	sfGFP, eGFP	[40,[42], [43], [44]]