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. 2020 Jul 30;5(4):252–267. doi: 10.1016/j.synbio.2020.07.006

Table 4.

Recent publications of extract preparation methods for non-E. coli prokaryotes and their reported GFP variantc yields from CFE reactions.

Organism Cell Growth Conditions (medium, temp., OD) Lysis Method Runoff/Dialysis GFP (ng/μL) Ref (s)
Bacillus megaterium 2xYT, 37 °C, OD 2.0 Sonication Y/N 134 [38]
Bacillus subtilus 2xYT-P, 30 °C, OD ~3 Sonication Y/Y 21.6 [39]
Bacillus subtilis 2xYT-P, 30 °C, OD 1.2–1.6 Sonication Y/Y 0.308 [40]
Clostridium autoethanogenum Gas with CO, 37 °C, ODb Sonication Y/Y 236 [47]
Corynebacterium glutamicum BHI, 30 °C, OD 1.2–1.6 Sonication Y/Y 3.32 [40]
Escherichia fergusonii 2xYT-P, 37 °C, OD 1.2–1.6 Sonication Y/Y 51.7 [40]
Klebsiella oxytoca 2xYT-P, 30 °C, OD 1.2–1.6 Sonication Y/Y 4.21 [40]
Lactococcus lactis MRS, 37 °C, OD 1.2–1.6 Sonication Y/Y 0.149 [40]
Pantoea agglomerans 2xYT-P, 30 °C, OD 1.2–1.6 Sonication Y/Y 172 [40]
Pseudomonas putida LB, 26 °C, OD 2.5 Sonication N/N 198 [45]
Pseudomonas putida 2xYT-P, 30 °C, OD 1.2–1.6 Sonication Y/Y 10.4 [40]
Salmonella enterica 2xYT-P, 37 °C, OD 1.2–1.6 Sonication Y/Y 0.263 [40]
Streptomyces coelicolorISP-5233 YEME, 30 °C, mid-log Sonication N/N 30a [142]
Streptomyces coelicolorISP-5233 YEME, 30 °C, mid-log Pressure N/N 30a [46]
Streptomyces coelicolorM1152 YEME, 30 °C, mid-log Pressure N/N 20a [46]
Streptomyces lividans66 YEME, 30 °C, mid-log Pressure N/N 45a [46]
Streptomyces lividans 66 YEME, 30 °C, mid-log Sonication N/N 84.7 [142]
Streptomyces lividans B12275 YEME, 30 °C, mid-log Pressure N/N 55a [46]
Streptomyces lividans B12275 YEME, 30 °C, mid-log Sonication N/N 117 [142]
Streptomyces rimosusB2659 YEME, 30 °C, mid-log Sonication N/N 100a [142]
Streptomyces roseosporus YEME, 30 °C, mid-log Sonication N/N 60a [142]
Streptomyces species F4474 YEME, 30 °C, mid-log Sonication N/N 40a [142]
Streptomyces venezuelaeDSM40230 GYM, 28 °C, OD 4.0 Sonication Y/N 35.1 [41]
Streptomyces venezuelae ATCC15439 YEME, 30 °C, mid-log Sonication N/N 45a [142]
Vibrio natriegens BHIN, 37 °C, log Pressure Y/Y 400 [43]
Vibrio natriegens BHI, 37 °C, OD 6.5–7.5 Sonication Y/N 1600 [44]
Vibrio natriegens LB + v2, 30 °C, OD 1.0 Sonication N/N 260 [42]
Vibrio natriegens BHI + v2, 37 °C, OD 1.2–1.6 Sonication Y/Y 78.5 [40]
a

Value was approximated from a figure in the corresponding reference.

b

OD value at harvest not reported.

c

Reported variants included eGFP, sfGFP, GFPmut3b, and GFP.