Figure 2.

N-Glycan Branching Reduces TLR4 and TLR2 Surface Levels by Promoting Endocytosis

(A–D) Flow cytometric analysis of B cells stimulated for 1–3 days and assessed for TLR4 and TLR2 surface expression (A), L-PHA binding (B), TLR4 and TLR2 surface-to-total ratio (C), and TLR4 and TLR2 endocytosis rates (D). Endocytosis rate over 1.5 h was calculated by dividing the MFI of acid-washed cells by the MFI of fluorescence-activated cell sorting (FACS) buffer-washed cells divided by 1.5 h (D). Data shown are mean ± SEM of cells stimulated in triplicate and representative of n ≥ 3 experiments. MFI, mean fluorescence intensity.