Figure 4.

N-Glycan Branching Deficiency in B Cells Promotes Pro-inflammatory APC Function

(A–F) LPS-stimulated B cells were co-cultured with allogeneic CD4⁺ T cells (A–C) or congenic 2D2 TCR transgenic CD4⁺ T cells +2.5 μg/mL hMOG_35-55 (D–F) under T_H1/T_H17/T_REG-inducing conditions. CD4⁺ T cells were assessed by flow cytometry for intracellular staining of IFNγ after 3 days (A and D) and IL-17A (B and E) and FOXP3 (C and F) after 4 days. Data shown are mean ± SEM of cells stimulated in triplicate and representative of n = 3 experiments. Unpaired one-tailed t tests. NS, not significant; ∗p < 0.05; ∗∗p < 0.01.