Figure 5.

N-Glycan Branching Deficiency in B Cells Promotes Inflammatory Demyelination

(A–C) EAE was induced in 8- to 12-week-old congenic C57BL/6 mice with day 0 indicating the time of immunization and clinical score (A), disease incidence (B), and highest clinical score (C) monitored over 35 days (n = 12 (F7,M5) CD19cre^+/-, n = 11 (F6,M5) Mgat2^f/fCD19cre^+/-).

(D and E) On day 35, mice induced with EAE were used for flow cytometric analysis of infiltrating CD4⁺ T cell and B220⁺ B cell in spinal cords (D) and in vivo cytokine production of splenic CD4⁺ T cells (E). Each symbol represents one mouse; horizontal line represents the mean.

Two-way ANOVA (A and B) and unpaired one-tailed t tests (C–E). NS, not significant; ∗p < 0.05; ∗∗p < 0.01.