FIGURE 6.

CREMSCLE can be used for co-expression of two proteins. (A,B) Two-photon z-projections of double labeled cells at 3 days post-electroporation. (A) pCAG-Cre (0.5 ng/μL) co-electroporated with pLNL-EGFP (1 μg/μL) and (A′) pCMV-mCherry (3 μg/μL) leads to (A′′) double labeling of sparse EGFP-expressing cells within a field of mCherry-labeled cells. Most cells labeled by EGFP are also positive for mCherry. (B) Co-electroporation of pCAG-Cre (0.1 ng/μL) with pLNL-EGFP (1 μg/μL) and (B′) pLNL-dsRed (3 μg/μL) results in nearly all cells expressing (B′′) both EGFP and dsRed by 3 days post-electroporation. (C) Number of cells per animal that expressed EGFP fluorescence (green), dsRed fluorescence (red), or both (yellow). By 2 days post-electroporation nearly every cell was double-labeled. n = 12 animals. Scale bar, 20 μm.