Fig. 4.

Blocking macrophage PI3K-AKT activation during ADM triggers reflux of inflammatory cells and tissue damage.

(A) Heat map lists normalized gene expression related to PI3K-AKT signaling, enriched in gene cluster 27. (B) The level of p-AKT in pancreatic macrophages on day 3 was analyzed by flow cytometry. (C) Relative expression of Pik3ca, Pik3cb, Pik3cd and Pik3cg in pancreatic acini (GSE126302) and pancreatic macrophage (GSE138134). (D) Mice were administrated with DMSO/TG100–115 (5 mg/kg, i.p., twice daily) since D2.5 post AP induction, and then harvested on D5. The level of p-AKT in pancreatic macrophage on day 3 were shown. (E) Representative H&E staining of pancreas (day 5) from DMSO- or TG100–115- treated mice. Scale bar,100 µm. (F) The proportion and number of CD11b⁺F4/80^mid, CD11b⁺F4/80⁻, CD11b⁺Ly6C^hi and CD11b⁺Ly6G⁺ cells from different treatment groups are shown. *P<0.05, **P<0.01, ***P<0.001, ns, not significant. Data presented as means ± SEM (unpaired two-tailed Student's t-test).