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. 2020 Jul 28;11:1726. doi: 10.3389/fmicb.2020.01726

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Copyright © 2020 Muchaamba, Eshwar, von Ah, Stevens and Tasara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Minimum-spanning tree illustrating the phylogenetic relationship based on cgMLST allelic profiles of the 2 PJI isolates (N843_10 and N843_15) and seven selected reference strains including three CC412 isolates (N12-1273, N18-2578 and N18-2708). Each circle represents an allelic profile based on sequence analysis of 1,701 cgMLST target genes. The numbers on the connecting lines illustrate the numbers of target genes with differing alleles. The different groups of strains are distinguished by the colors of the circles. Clonal strains (cutoff 10 allele difference) are shaded in light brown.