TABLE 1.
Strains and plasmids used in this study
| Strain ID | Description | References/Source |
| N843_10 | 2010 PJI isolate, serotype 1/2a, CC412 | This study |
| N843_15 | 2015 PJI isolate, serotype 1/2a, CC412 | This study |
| EGDe | Rabbit isolate and reference strain, serotype 1/2a, CC9 | Glaser et al., 2001 |
| N12-1273 | Sporadic human listeriosis isolate, serotype 1/2a, CC412 | Althaus et al., 2014 |
| LL195 | 1983 Swiss listeriosis outbreak, serotype 4b, CC1 | Bille, 1990 |
| N2306 | 2013-2014 Swiss listeriosis outbreak, serotype 4b, CC4 | Stephan et al., 2015 |
| J5051 | Listeria innocua used as anegative control | Guldimann et al., 2015 |
| N11-1850 | 2011 milk isolate, serotype 4b, CC217, used as positive control in biofilm assays | Ebner et al., 2015 |
| N843_10ΔrnjA | In-frame rnjA deletion | This study |
| N2306 ΔrnjA | In-frame rnjA deletion | This study |
| E. coli DH5α | Subcloning EfficiencyTM DH5αTM competent E. coli competent cells | InvitrogenTM |
| Florescent labeled Listeria strains | ||
| EGDe mO2FP | EGDe WT with pIMK-mO2fp integration into the tRNAArg locus | This study |
| N843_10 GFP | N843_10 with pPL3e-gfp integration into the tRNAArg locus | This study |
| N843_10 mO2FP | N843_10 with pIMK-mO2fp integration into the tRNAArg locus | This study |
| N843_15 mO2FP | N843_15 with pIMK-mO2fp integration into the tRNAArg locus | This study |
| N12-1273 mO2FP | N12-1273 WT with pIMK-mO2fp integration into the tRNAArg locus | This study |
| N2306 mO2FP | N2306 WT with pIMK-mO2fp integration into the tRNAArg locus | This study |
| L. innocua mO2FP | L. innocua with pIMK-mO2fp integration into the tRNAArg locus | Loessner Lab |
| N843_10ΔrnjA mO2FP | N843_10 ΔrnjA with pIMK-mO2fp integration into the tRNAArg locus | This study |
| N2306 ΔrnjA mO2FP | N2306 ΔrnjA with pIMK-mO2fp integration into the tRNAArg locus | This study |
| Plasmids | ||
| pKSV7 | Plasmid vector | Smith and Youngman, 1992 |
| pKSV7-ΔrnjA | Plasmid carrying construct for rnjA deletion | This study |
| pPL3e-gfp | Integrative plasmid vector pPL3e—gfp for the constitutive expression of green fluorescence protein (GFP) | Shen and Higgins, 2005 |
| pIMK-mO2fp | Integrative plasmid vector pIMK-mO2fp for the constitutive expression of mOrange2 fluorescence protein (mO2FP) | Loessner Lab |