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. 2020 May 4;10(3):561–579. doi: 10.1016/j.jcmgh.2020.04.014

Figure 1.

Figure 1

IL27 is secreted by macrophages/dendritic cells (DCs) in the gastric mucosa. (A) IL27 secretion measured by enzyme-linked immunosorbent assay from gastric lymph node cells (grey) or gastric mucosal immune cells (black) of TxA23 mice at 2 months of age. Each dot represents the data from 1 mouse, and samples from the same mouse are connected by a line. N = 5 mice per group from 2 separate experiments. Significance was determined using a Student t test. (B) Quantitative real-time PCR gene expression analysis for the 2 subunits of IL27 (Il27 and Ebi3) in sorted and stimulated immune and epithelial cells isolated from the gastric mucosa. Expression of target genes was relative to the Gapdh housekeeping gene. Statistical significance was determined using a Student t test. N = 3–5 mice per group from 3 separate experiments. (C) Representative flow cytometry plots showing the gating strategy for identifying the presence of macrophages (CD19-MHCII+CD11b+CD11c-F4/80+) and DCs (CD19-MHCII+CD11b-CD11c+) in the APC compartment of immune cells flushed from the gastric mucosa. ND, not determined.