Figure 7.

Control and Ebi3^-/- mice with autoimmune gastritis have similar immune cell numbers, proportions, and cytokine secretion profiles. (A) Total immune cell numbers isolated from the gastric lymph node (left) and the gastric mucosa (right). Each dot represents 1 mouse, with 15–20 mice per group. (B) Total numbers of immune cell subsets in control and Ebi3^-/- TxA23 mice, with 15–20 mice per group. (C) Representative flow cytometry histograms of IL27RA staining on different immune subsets (red) compared with IL27RA-deficient controls (gray), with 4–6 mice per group. (D) Analysis of cytokines secreted by stomach-infiltrating immune cells that have been restimulated in vitro with PMA/ionomycin for 72 hours. Supernatants were analyzed using a Milliplex bead-based cytokine assay (EMDMillipore; Burlington, MA). There were 3 mice per group. Significance was calculated using the Student t test. Ctrl, control; G-CSF, Granulocyte colony stimulating factor; IP-10, Interferon-induced protein 10; KC, Keratinocyte chemoattractant; LIF, Leukocyte inhibitory factor; LIX, LPS induced CXC chemokine; M-CSF, macrophage colony stimulating factor; MCP-1, monocyte chemoattractant protein 1; MIG, monokine induced by gamma interferon; MIP, macrophage inflammatory protein; PMA, Phorbol 12-myristate 13-acetate; RANTES, Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted; VEGF, vascular endothelial growth factor.