Figure 8.

IL27 acts on effector CD4 T cells to reduce inflammatory gene expression during autoimmune gastritis. Immune cells were flushed and sort-purified from the gastric mucosa of sham-treated and IL27-treated mice and subjected to scRNA sequencing analysis. (A) Uniform Manifold Approximation and Projection clustering of approximately 32,000 immune cells sequenced. Each dot is an individual cell. Cells are colored by cluster identity. (B) Il27ra expression in sequenced immune cell clusters. (C) Dot plot of the relative expression of all detected transcripts calculated from the per-cell average relative expression from effector CD4 T cells identified in the untreated and IL27-treated libraries. (D) Corresponding violin plots showing expression level of selected inflammatory genes in that effector CD4 T-cell subset. (E) Select genes significantly down-regulated by IL27 treatment in CD4+ T cells. MDSC, Myeloid derived suppressor cells; NK, natural killer; pDC, peripheral dendritic cells.