Table 3.
Function Parameters of Vital Organs (mean ± SD, n = 8)
| Groups | Parameter |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lung Function |
Liver Function |
Kidney Function |
Heart Function |
|||||||||||
| Inspiratory Resistance (Ri, cm H2O/mL.s) | Expiratory Resistance (Re, cm H2O/mL.s) | Compliance (Cl, mL/cm H2O) | The Peak Expiratory Flow (PEF, mL/min) | Maximal Voluntary Ventilation (MVV, mL) | ALT (U/L) | AST (U/L) | Urea (mmol/L) | Crea (mmol/L) | Troponin (pg/mL) | CK (U/L) | CK-MB (U/L) | LDH (U/L) | HBDH (U/L) | |
| Sham | 1.80 ± 0.09 | 1.37 ± 0.05 | 0.28 ± 0.02 | 38.09 ± 1.67 | 194.46 ± 3.27 | 39.51 ± 3.73 | 133.49 ± 32.18 | 4.55 ± 0.64 | 16.5 ± 0.71 | 210.24 ± 11.57 | 1,439.5 ± 140.12 | 1,520.5 ± 186.34 | 1,402 ± 138.63 | 469.50 ± 60.93 |
| Burn | 5.45 ± 0.13 ∗∗ |
4.48 ± 0.09 ∗∗ |
0.11 ± 0.03 ∗ |
20.24 ± 2.04 ∗∗ |
121.07 ± 2.38 ∗∗ |
210.33 ± 8.57 ∗∗ |
892.41 ± 56.33 ∗∗ |
13.63 ± 0.71 ∗∗ |
27.48 ± 0.63 ∗∗ |
320.78 ± 15.35 ∗∗ |
5,741.26 ± 137.51 ∗∗ |
3,620.97 ± 91.29 ∗∗ |
3,431.17 ± 165.74 ∗∗ |
1,183.82 ± 166.27 ∗∗ |
| Burn + hMSC | 3.31 ± 0.40 @@ |
2.70 ± 0.38 @@ |
0.20 ± 0.02 @ |
31.72 ± 1.15 @@ |
163.96 ± 1.85 @@ |
113.10 ± 4.18 @@ |
542.39 ± 20.05 @@ |
8.59 ± 0.50 @@ |
22.18 ± 0.77 @ |
259.39 ± 8.70 @@ |
2,952.80 ± 73.15 @@ |
2,490.38 ± 56.64 @@ |
2,172.86 ± 72.10 @@ |
721.95 ± 38.17 @@ |
| Burn + mock | 3.54 ± 0.18 @@ |
2.99 ± 0.17 @@ |
0.20 ± 0.04 | 31.38 ± 0.93 @@ |
163..81 ± 2.04 @@ |
114.67 ± 9.36 @@ |
544.85 ± 34.41 @@ |
8.76 ± 0.88 @@ |
22.96 ± 0.49 @ |
261.46 ± 9.98 @@ |
2,967.96 ± 62.97 @@ |
2,518.10 ± 90.01 @@ |
2,221.33 ± 85.71 @@ |
742.92 ± 41.24 @@ |
| Burn + IGF-o | 2.19 ± 0.17 # |
1.84 ± 0.56 # |
0.26 ± 0.02 | 36.49 ± 0.68 # |
182.75 ± 3.66 ## |
68.72 ± 6.35 ## |
357.33 ± 28.80 ## |
5.94 ± 0.27 ## |
18.01 ± 0.36 # |
229.32 ± 10.27 # |
2054.76 ± 90.29 ## |
1903.48 ± 103.4 ## |
1,696.57 ± 68.84 ## |
585.71 ± 18.48 ## |
| Burn + IGF-i | 4.73 ± 0.20 ## |
4.17 ± 0.38 ## |
0.13 ± 0.05 | 23.57 ± 0.51 # |
132.07 ± 2.03 ## |
187.27 ± 9.59 ## |
825.48 ± 36.10 ## |
11.09 ± 0.45 # |
25.51 ± 0.14 # |
294.21 ± 13.52 # |
4,562.19 ± 115.26 ## |
3,360.51 ± 57.32 ## |
3,074.95 ± 109.12 ## |
1,003.92 ± 42.77 ## |
| Burn + miR-301a-3p-o | 4.28 ± 0.11 ## |
3.86 ± 0.20 ## |
0.14 ± 0.03 | 24.49 ± 1.25 # |
136.71 ± 3.12 ## |
178.68 ± 7.44 ## |
784.52 ± 20.45 ## |
10.54 ± 0.62 # |
24.82 ± 0.53 # |
289.65 ± 3.26 # |
4,357.34 ± 197.05 ## |
3,283.94 ± 45.15 ## |
2,918.33 ± 70.31 ## |
984 ± 25.91 ## |
| Burn + miR-301a-3p-i | 2.55 ± 0.16 # |
2.05 ± 0.23 # |
0.25 ± 0.04 | 35.72 ± 1.03 | 179.96 ± 1.94 ## |
75.35 ± 3.82 ## |
371.19 ± 26.71 ## |
6.34 ± 0.19 ## |
19.15 ± 0.79 # |
234.05 ± 9.51 # |
2,211.38 ± 79.67 ## |
2075.37 ± 42.71 ## |
1743.22 ± 91.29 ## |
610.11 ± 20.10 ## |
| miR-301a3p-i +IGF-1-i |
3.09 ± 0.25 & |
2.52 ± 0.24 & |
0.22 ± 0.03 | 32.02 ± 1.09 | 167.24 ± 1.51 & |
100.72 ± 13.59 && |
481.48 ± 29.28 && |
7.94 ± 0.52 & |
21.95 ± 0.54 & |
250.29 ± 4.03 & |
2,708.84 ± 35.44 && |
2,326.95 ± 76.74 & |
2075.02 ± 79.63 && |
697.73 ± 26.38 & |
MiR-301a-3p negatively regulating hMSC-secreted IGF-1 improves function parameters of vital organs in severely burned rats (mean ± SD, n = 8). ALT, alanine transaminase; AST, aspartate transaminase, Crea, creatinine; CK, creatine kinase; CK-MB, MB isoform of CK; LDH, lactate dehydrogenase; HBDH, a-hydroxybutyrate dehydrogenase. ∗p < 0.05 and ∗∗p < 0.01 compared with sham group, respectively. @p < 0.05 and @p < 0.01 compared with burn group, respectively. #p < 0.05 and #p < 0.01 compared with burn + mock group, respectively. &p < 0.05 and &&p < 0.01 compared with burn + miR-301a-3p-i group, respectively.