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. 2020 May 23;10(3):581–599. doi: 10.1016/j.jcmgh.2020.05.004

Figure 3.

Figure 3

Gli1+fibroblasts lineage-traced before pancreatitis-induced PanIN lesion formation contribute to the stroma. (A) Genetic scheme for a KF;Gli1CreERT/+;RYFP mouse. (B) Experimental design for labeling healthy Gli1+ cells before PanIN generation. Adult mice 5–8 weeks old were given 5 tamoxifen gavages and rested for a week before 2 days of cerulein injections to induce pancreatitis. After 3 weeks, samples were collected. (C) IHC staining for YFP in KF;Gli1CreERT/+;RYFP and KF control tissue (inset) labeled before PanIN generation. Scale bar: 100 um. (D) Quantification of YFP+ staining area from IHC (n ≥ 8) (E) IF staining on KF;Gli1CreERT/+;RYFP and KF control (inset) samples of YFP (green), PDGFRβ (red), cytokeratin 19 (CK19) (white), and 4′,6-diamidino-2-phenylindole (DAPI) (blue); YFP (green), αSMA (red), amylase (pink), and DAPI (blue); YFP (green), podoplanin (red), E-cadherin (white), and DAPI (blue); and YFP (green), CD105 (red), and DAPI (blue). Scale bar: 50 um. (F) A representative YFP IHC image of a Gli1CreERT/+;RYFP mouse that followed the same labeling scheme. Scale bar: 100 um. AMY, amylase; ECAD, E-cadherin; TAM, tamoxifen; WT, wild-type.