Figure 2:

(A) The mycobacterial burden at day 30-post low dose aerosol infection with M. tuberculosis H37Rv in the lungs and spleens of C57BL/6 mice inoculated via the intranasal route with CpG-A, -B, and -C subtypes formulated with liposome and ESAT-6 three times at two-week intervals (N = 5 mice per group). (B) TNF-α, IFN-γ and IL-2 spot forming units (SFU) in spleen cells generated at day 30 post-vaccination with CpG-C based vaccine as shown by ELISpot assay. (C) Mice were inoculated with CpG-C formulated with liposomes and ESAT-6 and inoculated via the intragastric (i.g.), subcutaneous (s.c.), intraperitoneal (i.p.), and intranasal (i.n.) route as described above, rested for 30 days and then infected with M. tuberculosis H37Rv. Log10 CFU was determined 30 days post-infection (N = 3–5 mice per group). The Log₁₀-transformed CFU data was analyzed using the One-way ANOVA for independent measures with post-hoc Tukey test, and SFU data were analyzed using the Student t-test with each treatment group being compared to the Saline-treated group. * = p<0.05, ** = p<0.01, *** = p<0.001