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. 2020 Jul 28;10:1234. doi: 10.3389/fonc.2020.01234

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Copyright © 2020 Xie, Tang, Pang, Xu, Yang, Wang, Huang, Huang, Liu, Tong, Zhang, Wang, Zhang, Lan, Liu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LSD1 regulates BCa EMT in vivo and in vitro. (A–C) T24 cells with or without LSD1 knockdown were seeded in 6-well-plates and cultured for 48 h; mRNA and protein levels of EMT markers E-Cadherin, N-Cadherin, Vimentin, and Twist were determined by quantitative RT-PCR and Western blotting. The data represent means ± S.D. *P < 0.05. (D) Lungs collected from T24 cells with or without LSD1 knockdown tail vein injected model of nude mice were conducted IHC staining with antibodies against E-cadherin, N-cadherin, Vimentin and Twist. (E) Tumors collected from T24 cells with or without LSD1 knockdown subcutaneous xenograft model of nude mice treated as indicated were conducted IHC staining with antibodies against E-cadherin, N-cadherin, Vimentin, and Twist.