Figure 5.

Inhibiting LSD1 attenuates BCa cell proliferation in vivo. (A,B) T24 and BIU87 cells with or without GSK were seeded in 96-well-plates with 1 × 10³ cells per well in growth media cultured for 7 days. Cell viabilities were estimated by CCK8 every other day. (C–H) Nude mice bearing T24 cell-derived or patient-derived xenograft (PDX) metastatic bladder cancer tissue was treated with or without GSK2879552 for 4 weeks; the tumors were measured and weighed. The data represent means ± S.D. *P < 0.05. (I) Xenografts collected were conducted IHC staining with antibodies against Ki67 (proliferation marker) and Bcl-2 (apoptosis marker). (J) Patient-derived xenograft (collected were conducted IHC staining with antibodies against Ki67 and Bcl-2. (K) Schematic model of the hypothesized mechanism by which LSD1 promotes EMT development.