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. 2020 Jul 28;11:818. doi: 10.3389/fgene.2020.00818

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Copyright © 2020 Lou, Wei, Xiao, Huo, Hong, Sun, Shuai and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Nano-SiO₂ on the expression of DNA methylation-associated proteins in human bronchial epithelial cells. DNMTs activity of 16HBE cells (A) and BEAS-2B cells (B) treated with Nano-SiO₂ for various durations were determined. The data are shown as mean ± SD (n = 3). *p < 0.05, compared with the control group (P0). A549, an epithelial cell line derived from human lung carcinoma, was used as a positive control. Levels of DNMT-1, DNMT-3a, DNMT-3b, MeCP-2, and MBD-2 in 16HBE cells (C) and BEAS-2B cells (D) treated with Nano-SiO2 for different passages were evaluated using western blot. GAPDH was used as the loading control.