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. 2020 Jul 28;8:572. doi: 10.3389/fcell.2020.00572

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Copyright © 2020 Hampl, Dumkova, Kavkova, Dosedelova, Bryjova, Zahradnicek, Pyszko, Macholan, Zikmund, Kaiser and Buchtova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of SHH ligand polarized localization in the rostral area of the palatal shelves during pre-hatching development. Immunohistochemical detection of SHH polarized localization in mesenchymal cells of the developing palatal shelves. Pictures (A–C) show transversal sections divided into ventral, middle and dorsal areas. Rose dot plots of 77 dpo (A^v,A^m,A^d), 98 dpo (B^v,B^m,B^d), and 105 dpo (C^v,C^m,C^d) stages show polarity of SHH in individual cells (each dot) according to the dorsoventral and mediolateral axes. The 360 degree circle was divided into 12 zones each with a 30 degree span. 0°, medial direction; 90°, dorsal direction; 180°, lateral direction; 270°, ventral direction. n, number of cells analyzed in each area. Scale bar: 50 μm.