FIGURE 4.

Ossification of individual bones contributing to the secondary palate during pre-hatching stages. Lower power images (A–D) of palatal view of all bones and cartilages that contribute to formation of palate at earlier pre-hatching stages of chameleon embryos. Higher power images display either the palatine bones (A′–D′) or pterygoid bones (A″–D″) ossification during pre-hatching development of the veiled chameleon. The same arrangement of pictures for later stages of pre-hatching stages of chameleon embryos (E–H″). Bones and cartilage were stained using Alcian blue and Alizarin red staining. bo, basioccipital bone; bs, basisphenoid bone; ec, ectopterygoid bone; et, egg tooth; j, jugal bone; mx, maxillary bone; pl, palatine bone; pmx, premaxillary bone; pof, post-orbitofrontal bone; pt, pterygoid bone; q, quadrate bone; sq, squamosal bone; v, vomer. Scale bar: 1 mm.