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. 2020 Jul 28;8:572. doi: 10.3389/fcell.2020.00572

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Copyright © 2020 Hampl, Dumkova, Kavkova, Dosedelova, Bryjova, Zahradnicek, Pyszko, Macholan, Zikmund, Kaiser and Buchtova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of proliferating cells in the palatal shelves of chameleon embryos. Cell proliferation in the palatal shelves of 77 dpo (A–D″), 98 dpo (B–E″), and 105 dpo (C–F″) stages of the veiled chameleon. Hematoxylin-Eosin staining on the frontal head sections in the lower power and higher power view from the rostral (A,A′–C,C′) and caudal (D,D′–F,F′) part of the palatal shelves. Immunohistochemical detection of PCNA-positive cells in the higher power view (details from black rectangles) from the rostral (A″–C″) and caudal (D″–F″) palatal shelves. PCNA-positive cells – green nuclei, PCNA-negative cells – blue nuclei (DAPI). White arrows point regions of PCNA-expressing cells in the forming palatal shelves. dpo, days post oviposition; mx, maxillary bone; ne, nasal epithelium; oe, oral epithelium; pl, palatine bone; ps, palatal shelf. Scale bar: 200 μm.