FIGURE 9.

SHH and acetylated α-tubulin protein localization in the rostral palatal shelves in pre-hatching chameleons. Immunodetection of SHH (red) and acetylated α-tubulin (green) proteins on transversal sections. Lower power pictures overview localization at 77 dpo (A), 98 dpo (B), and 105 dpo (C) during pre-hatching development. White rectangles define regions focused on ventral (v), middle (m) and dorsal (d) parts of the palatal shelves. Pictures (A^v–C^d) show higher power details from ventral, middle and dorsal regions. White arrowheads indicate polarized colocalization of SHH and primary cilium (detected using acetylated α-tubulin) on the same cellular side. Acetylated α-tubulin is present not only in primary cilia, but as well in microtubules of mitotic spindle, therefore there was signal in both structures detected in green. Nuclei are counterstained with DRAQ5. Scale bar: 20 μm.