Figure 5.

FECH Inhibition Caused a Decrease in Glycolytic Function

(A–H) (A and E) ECAR kinetic traces for HRECs under FECH knockdown or NMPP chemical inhibition. (B and F) Glycolysis, (C and G) glycolytic capacity, and (D and H) glycolytic reserve were calculated based on ECAR curves for the respective treatment group.

(A and E) Representative ECAR curve of three individual experiments. Bar graphs indicate mean ± SEM, n = 3; ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, unpaired Student's t test. 2-DG, 2-deoxyglucose.