FECH Inhibition In Vivo Decreased Mitochondrial Respiration in Retina
(A) Representative OCR kinetic traces for retina from animals treated with NMPP.
(B–D) (B) Basal respiration, (C) maximal respiration, and (D) spare respiratory capacity were calculated based on OCR curves for the respective treatment groups.
(E) Immunoblot showing CcO nuclear encoded subunit 1 (COX4I1) protein expression from three pooled retinal tissue lysates from NMPP treated eyes and (F) quantification of immunoblots. Graphs indicate mean ± SEM for two tissue punches from each retina per treatment group, n = 6–7 per treatment condition. ∗p < 0.05, ∗∗∗p < 0.001, unpaired Student's t test.