Table 2. List of primers used for real-time PCR.
Target | Primer name | Oligonucleotide sequence |
---|---|---|
All bacteria | 341f | CCTACGGGAGGCAGCAG |
534r | ATTACCGCGGCTGCTGG | |
Bifidobacterium spp. | BifiLM26F | GATTCTGGCTCAGGATGAACGC |
Bif228R | CTGATAGGACGCGACCCCAT | |
Lactobacillus spp. | LactoR’F | CACAATGGACGMAAGTCTGATG |
LBFR | CGCCACTGGTGTTCTTCCAT | |
Clostridium cluster XIV | CXIV-F1 | GAWGAAGTATYTCGGTATGT |
CXIV-R2 | CTACGCWCCCTTTACAC | |
Clostridium perfringens | Cperf 165F | CGCATAACGTTGAAAGATGG |
Cperf269R | CCTTGGTAGGCCGTTACCC |